Aim: was to assess knowledge about hepatitis C virus infection at selected theoretical colleges' students at Assiut University and suggest instruction brochure. Research design: A cross-sectional survey based on a self-administered questionnaire. Setting: The study was conducted at selected theoretical faculties (Faculty of Law, Commerce, and Art). Samples: The study sample consisted of (1000) adult students attending faculties of Law, Commerce, and Art. Age from 18 to 24 years, study years from 1st until 4th study years, males and females. Tools: Self-structured questionnaire sheet: It consisted of two parts: Socio demographic data & Part (2): Assessment of students' knowledge about HCV developed and provided brochure for students about HCV. Results: The highest percentage of the sample (number of students) equally from law and commerce were (40%) from each faculty. Concerning gender; females (73.5%) from 1st until 4th class (25%) in each class. More than half of sample (52.2%) had poor knowledge level about hepatitis C virus. Conclusion: The majorities of samples who had good knowledge level were females, fourth class, and from urban area. Recommendation: Sufficient relevant suggested instruction brochure & health instruction programs to facilitate educating students in theoretical colleges about mode of transmission and prevention of hepatitis C.