Acute kidney injury (AKI) is a common and serious problem affecting millions and causing death and disability for many people. Aim of the study was to evaluate the effect of implementing fluid therapy protocol for pregnant and postpartum women outcomes who at risk for developing acute kidney injury. Patients and Methods: Quasi experimental research design was used to conduct this study; the study was carried out in Obstetric ICU at Assiut University Hospital. The study sample consisted of intervention group (15 patients) who received fluid therapy protocol and control group (15 patients) who received only the routine hospital care, the study was conducted in 6 months. Tools: first tool "Renal impairment assessment sheet", second tool "Protocol of fluid flow chart ", third tool "Patients' outcomes evaluation sheet". Results: No patients in the intervention group developed acute kidney injury but 60% of control group developed AKI with a statistical significant difference between intervention and control groups in relation to developing AKI (P=0.001). Conclusion: Implementing fluid therapy protocol was significantly effective in preventing developing AKI among at risk pregnant and postpartum women. Recommendations: Emphasize on the importance of accurate monitoring of urine output and CVP in obstetric patients who at risk for developing AKI in detection and preventing any problem or deterioration.