Assessment of Nurses performance Regarding Infection Control in Operating Room At main Assiut University and AlEman Hospital (Suggested guidelines). Aim: Assess of nurses performance regarding infection control in operating room at main Assiut University and Al Eman Hospitals (Suggested guidelines(. Subject and methods: the study was conducted in orthopedic and general surgical department. Study design: Descriptive design was utilized. Tools: (1) Nurses knowledge questionnaire sheet, (2)Nurses practice observation checklist and development of booklet guidelines of infection control in operating room. Results: majority of nurses had good knowledge about the infection control in operating room at both hospitals. Nurses at AL Eman Hospital had unsatisfactory level of practice, half of nurses at Main Assiut University Hospital had unsatisfactory level of practice. Conclusions: nurses in operating room at both hospital have good knowledge regarding infection control in operating room, all nurses at AL Eman hospital had unsatisfactory level of practice of the principle of infection control in operating room. While half of nurses at Main Assiut University hospital had unsatisfactory level of practice.. Recommendations: the study recommended that, continued education training programs for nurses, receive vaccination before started work, activating the role of infection control team