Liver biopsy has been an integral part of the management of patients with chronic viral hepatitis. Aims of this study; to assess patient's knowledge about liver biopsy, the effect of implementing suggested nursing teaching guidelines for patients undergoing liver biopsy on their outcome and to evaluate the effect of implementing suggested nursing teaching guidelines for patients undergoing liver biopsy on their outcome. Subjects and Methods:The study was conducted in the Tropical Medicine and Gastroenterology Department and outpatient hepatitis viruses clinic at AssiuUniversity Hospital. Study design:Quasi experimental research design was utilized in this study. The subjects of this study consisted of 200 male and female adult patients.Tool:Structured Interview questionnaire sheet.Nursing guidelines for Patients undergoing liver biopsy. Results:The majority of studied patients were male(85.5%), ( 72%) were married, ( 45%) illiterate. The results also revealed that majority of the studied patients had poor level guidelines about liver biopsy with percentage (93.5%). Conclusion:All studied patients weren't properly prepared prior before taking liver biopsy.Recommendation, further research studies are needed to focus on more nursing guidelines was developed according to the patients' needs that can help patients in provision of a safe and effective care for liver biopsy and improvement patient outcome