Organ transplant in Egypt presents difficult ethical and legal challenges for both the transplant community and society. Aim of the study: Evaluate the level of knowledge toward organ donation and transplantation among Assiut University students and assess their opinions about organ donation. Subjects and Method: Descriptive research design was used in this study. The study included (1075) students, were selected randomly from six facilities at Assiut University. through a period extended from February (2013) to the end of April (2013). Data of this study was collected by using self-administered questionnaire. Results: The mean age of students ± SD was (20.02 ± 1.45). Less than two thirds of them were females and live in a rural area. (53.8%) of students from non health faculties. There was a statistically significant difference between students' age, sex, faculty type and grades at college, with their knowledge about organ donation and transplantation. Conclusion: less than two thirds of students had poor knowledge about organ donation and transplantation, and the majority of them had negative opinions about it. Recommendations: Improve students' awareness by conducting ongoing scientific seminars for students and provide brochures on this subject in the university library.