Background: Learning style of students is one most important factors which influence their learning. There is a relationship between learning styles and students' academic achievements. The aim of the study: To determine the learning styles preferred by the faculty of nursing students at Assiut University and its impact on their academic achievement. Subject and method: A descriptive correlational research design used to conduct this study. A total number of studied sample were (n= 641): first (n= 148), second (n= 217), third (n= 153) and fourth (n= 123) year. Data collection tool consisted of three parts: I-Socio-demographic data sheet, II-Learning styles questionnaire sheet, and III-Student academic achievement grade. Results: There was a positive strong correlation between preferred learning style in the first, second and fourth year and their academic achievement. While, there was a positive moderate correlation in the third year. Conclusion: The highest percentage of the study sample were preferred Uni – mode learning styles. Overall, the combination of learning style has a greater impact on their academic achievement than use one preferred learning style. Recommendations: Encourages nursing students to use all learning styles. Conduct a workshop for nursing educators about "the importance of learning styles to improve learning and teaching process"