Egypt is one of the countries in the world where the problem of obesity has been nearing an epidemic level. Currently, nearly 70% of adult women and 48% of men in Egypt are overweight or obese. This study aimed to evaluate the impact of the health education programme to control obesity among female employees at Assiut University. A quiz-experimental design was used to apply health education intervention. A convenient sampling technique was utilized. Data was collected using two tools, the first tool, self administered questionnaire it cover two parts, part one included socio-demographic. Part two consisted female employees' knowledge about nutrition and obesity) the second tool Anthropometric measurements (waist circumference weight, height, and BMI). The mean age was 45.60 ± 9.65. The results illustrated that about three quarters (74.0%) of the studied sample has poor knowledge in pre-test compares to only (12.0%) in post-test had poor knowledge. Also more than one quarter (26.0%) of the studied sample had satisfactory level of knowledge in pre-test, while in post-test more than half (54.0%) of them have the satisfactory level of knowledge. There is a significant difference in pre/post tests of female employees' knowledge. Conclusion the health education program has an effective role in improvement of knowledge about balanced diet and obesity. Also, recommended that Booklets of Arabic language about obesity and management of body weight should be available in library of faculties. Increase public awareness about balanced diet, nutritional needs in every age group.