Background: The need for nursing is a universal issue. Nurses' awareness of their rights and responsibilities make them more satisfied and provide high quality care. Aim: Explore nurses' awareness of their rights and responsibilities at Sohag City Hospitals. Research design: A descriptive research design was used in the present study. Setting: This study was conducted at Sohag University Hospital and Sohag General Hospital. Sample: It was (365) nurse from Sohag University Hospital & (175) from Sohag General Hospital Results: Reveals that, the highest percentage of nurses has high level of awareness as regard to their rights (81.6% & 86.9%) respectively, and responsibilities (83.8% & 90.9%) respectively, at both Sohag University and Sohag General Hospitals. Conclusion: There was a positive correlation between age and nurses' awareness about their rights and responsibilities at both Sohag University and Sohag General Hospitals. Recommendations: Every hospital should make a meeting for newly employed nursing personnel to inform them of their Code of ethics, researches should be done to evaluate nursing practice of their rights and responsibilities