Background: Postpartum hemorrhage remains one of the major causes of maternal death that occurs worldwide which can be preventable and treatable. Aims of the Study: Assess of maternity nurses knowledge about preventive and therapeutic measures of postpartum hemorrhage. The effect of teaching program about preventive and therapeutic measures of postpartum hemorrhage on improving maternity nurses knowledge through implementation development. Setting: In woman's health university and El-Mabara hospital at Assiut city. Design: A quasi experimental (pre/post – test) has been used in this study. Sample: A convenient sample (62) has been used (25) of them from woman health hospital, (37) of them from El-Mabara hospital. Tools: Two tools were used (1) self-administrative questionnaire has been used to collect data. (2) Teaching program with booklet. Results: The findings reported that more than half of them aged 30 years or more and had secondary education, Most of them had unsatisfactory knowledge about the prevention of postpartum hemorrhage. But, unsatisfactory this knowledge has been improved after program implemented. Conclusion: Nurses had unsatisfactory knowledge regarding preventive and therapeutic measures of postpartum hemorrhage in the pre-test. The educational program was successful in achieving its goal of upgrading or improving their knowledge. Recommendations: The study recommends focuses on establishing plans for periodical and scheduled training courses and educational programs for nurses