Aim: The aim was to audit and re-audit nursing care for children undergoing central venous line insertion in Pediatric Intensive Care Unit. Design: A quasi-experimental research design was used for the present study. Setting: The study was carried out at Pediatric Intensive Care Unit, Assiut University Children Hospital. Subjects: Convenience sample was obtained from nurses who gave care to all cases undergoing CVC insertion, working at the previous setting (n= 33). Data Collection Tools: Two tools were used: A structured interview questionnaire sheet and an indirect observation checklist. Results: The study revealed that a mild statistically significance differences were detected as regards CVC Insertion and medication administration (p=0.373 and 0.073 respectively), while the nurses' unsatisfactory practice concerning heparanization and dressing change declined during the re-auditing (p=0.208 and 0.422 respectively).Conclusion: Re-auditing of nurses caring for children undergoing central venous line insertion improved more significantly than auditing. Recommendations: Pediatric nurses should update their knowledge and practice through continued nursing education, training, and frequently attending seminars and conferences especially in infection control skills.