Background: Acute pancreatitis is the inflammation of the pancreas that results in auto-digestion by its own pancreatic enzymes. The educational program is considered to be an important means to provide nurses with theoretical and technical information needed to acquire skills and competencies necessary to continually improve nursing practice. Objective: Theaim of this study was to evaluate effectof educational program on nurses 'performance regarding patients with acute pancreatitis. Design: A quasi-experimental research design was used to conduct this research. Setting: The study was conducted in intensive care unit at Assiut University Rajhy Hospital for Liver. Subjects: All available nurses about 40 nurses. Tools: Data were collected using: tool1 nurse's knowledge assessment questionnaire, tool II nurse's observation checklist. The Results revealed that majority of the nurses their age less than 40 years old, with mean age of 25.98±6.46, female, where about 52.5% of study nurses had married. The majority of nurses had unsatisfactory total knowledge pre education program. It improved immediately post education program. Conclusion: The study concluded that the education program lead to significant improvement in nurses' knowledge and practice about acute pancreatitis. Recommendation: Continues educational nursingprogram about care of acute pancreatitis patients.