The present study aimed to evaluate the potentially protective effects of aqueous extract of tamarind fruit pulp (AETF) against hyperglycemia and male infertility in diabetic rats. AETF was getting by soaking tamarind fruit pulp in distilled water in a ratio of 1:2 (fruit pulp: water) for 12 hr. After soaking period, the solution heating for 20 min at 50º C, then filtered and concentrated under vacuum. In the present study, thirty-five male albino rats were used and divided into five groups (each of= 7 rats). Group 1 served as normal rats, group 2 served as untreated diabetic rats; and diabetic groups 3, 4, and 5 were given orally concentrated AETF at a dose of 1,2 and 3ml/100g body weight/day, respectively. The results revealed that, oral administration of AETF for diabetic rats significantly (P < 0.05) improved testes, seminal vesicles and prostate glands weight, compared with untreated diabetic rats. The treated diabetic rats had a significant lower (P < 0.05) in BGL, serum activities of AST, ALT and ALP enzymes; and larger in serum insulin, testosterone, LH and FSH levels, compared to untreated diabetic rats. In the testes tissues, AETF significantly ameliorated MDA level and activities of GSH, GPx, SOD and CAT enzymes in diabetic rats. Histopathological investigation for testes of tread diabetic rats showed almost normal histological structure of seminiferous tubules with the lumen filled of spermatozoa, especially with higher level of AETF. In conclusion, AETF exhibited hypoglycemia, antioxidant activity and protective effect against male infertility in diabetic rats.