Aluminum sulfate is known to create toxic effects on several systems of experimental animals. This research was undertaken to examine the absorption of aluminum confirmed by histological examination of rat´s organ after single intraperitoneal (IP) injection of AL2 (So4) 3. Male and female rats received a single (IP) injections of (23, 67, 200, 600, 1800, 5400, 16200, 48600) mg/kg b.wt. AL2 (So4) 3 dissolved in distilled water (n=6/sex/group). After 14 days male and female tissues were collected for aluminum (AL) content and histological damage assay. Its effect was evaluated in liver, kidney, lungs, spleens, heart, stomach, intestine, brain, testis, ovary and uterus compared to those of control rats. No mortality was recorded during the two weeks. Injection of higher doses of AL2 (So4) 3 produced burning of skin around the injection area for both male and female rats. Rats treated with (1800, 5400, 16200, 48600) mg/kg b.wt. AL2 (So4) 3 s showed a significant increase of aluminum accumulation and histological change in male and female rats, stomach, small intestine, liver, and lung. short term use of a low dose of AL2 (So4) 3 is safe and induce mild histopathological alteration for the different body organs. On the other hand, severe histopathological alteration synchronized with aluminum accumulation in the body organs are induced by using a single higher dose of AL2 (So4)3.