Molecular diagnosis represents one of the important trends in the confirmation of morphological identification to the root-knot nematodes which one of the most global pathogens. In this context, current study aimed to describe Meloidogyne javanica occurring in cucumber plants in Assiut governorate (Upper Egypt) using the random amplified polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) assay. Four mitochondrial and specific-specific sequences characterized amplified regions (SCAR) primers were used. The results showed that the SCAR primers, Fjav/Rjav and Far/Rar were efficiently amplified with 670 bp and 517 bp, respectively. On the contrary, the mitochondrial primers did not show any results. In conclusion, the present study recommended the SCAR primers as effective identified primers for the species of Meloidogyne javanica.