Punching shear failure in flat plate slabs is considered as a vital topic to investigate. The fact that this failure is brittle makes it an essential field to study. Many researches have been carried out to investigate the punching shear of flat plate slabs. Nevertheless, using prestressing in enhancing punching behavior wasn't of such a big contribution. An experimental study for enhancing punching shear volume of interior slab-column links in flat plate slabs by prestressing is presented through a parametric study in this research. The parameters taken into consideration in this study are prestressed vertical studs of different number of rows. Through the experimental program, deformation features, the load carrying capacity, and the cracking patterns have been examined. A comparative study between the behavior of the improved slabs and their controls has been made. This study showed that the suggested system might be of a good benefit in practice to be used. A comparison between the experimental punching load and the calculated using various design codes has also been made. It offered acceptable agreement.