A total of 60 specimens were collected from superficial mycosis (50 from humans and 10 from pet animals). Human cases were recruited to a private laboratory for mycological examination in Cairo while samples from pet animals were collected from veterinary private clinics in Zagazig, Damietta and Cairo in the period of 2019-2021. All samples were subjected to mycological examination including KOH, direct microscopy, and isolation of causative agents. Concerning human samples, the Dermatophytes were isolated from 46% of the samples where T. rubrum and T. mentagrophytes were recovered from Tinea corporis and Tinea pedis cases in respect, followed by M. canis and T. violaceum. From Tinea capitis, M. canis and T. violaceum dominated the scene. Non-dermatophytes were isolated from 27% of the samples mainly from onychomycosis and the recovered isolates were Aspergillus (A) nidulans, A. flavus, A. niger followed by Fusarium, acremonium and Chrysosporium. Yeast isolates were obtained from 27% of Onychomycosis and Tinea pedis cases and were represented by C. albicans followed by C. tropicalis, C. parapsilosis and C. krusei. From pet animals, dermatophytes incidence was 57% where M. canis was obtained from ringworm cases. C. albicans was recovered from 29% of the samples while the only isolated non-dermatophyte was Scopulariopsis brevicaulis (from 14% of the total samples)