Gallibacterium anatis is a widely distributed species of bacteria isolated from birds. The purpose of this preliminary investigation is to detect the prevalence of G. anatis infection in ill hens from private commercial broiler farms in Egypt's ELsharkia governorate. A total of 80 tracheal swabs were collected from broiler chickens with respiratory symptoms. Bacteriological investigation of the collected specimens revealed a prevalence of G.anatis of 35%. All isolated bacteria passed the oxidase, catalase, nitrate reduction, sucrose, sorbitol, and mannitol fermentation tests. However, the acquired isolates failed to ferment citrate, indole, urease, gelatinase, or maltose. Additionally, the recovered isolates failed the motility test. The gold standard method of bacterial isolation and identification (which includes culture characteristics, hemolytic activity on blood agar, morphological characteristics, motility test, and biochemical characteristics) is critical for determining the presence of G. anatis in birds.