The genus Helicobacter contains more than 35 species, Gram- negative microaerophilic bacteria, which colonizes the gastrointestinal tracts of humans and different animals. aim of work transfer to end of introduction to determine the susceptibility of H. pylori isolates to several antimicrobial agents to overcome the H. pylori treatment failure. For this purpose, 25stool sample from dog, 20 stool samples from cat and 75 stool samples from their human owners were collected for H. pylori infection. H. pylori was detected by culture in 36%, 20% and 41.3% of dog, Cat and human samples, respectively. Antimicrobial resistance of H. pylori in human, dog and cat to ceftazidime is100% .to amoxicillin is 76%, 100% and 100% respectively, to bacitracin is 88%, 100% and 100% respectively, to erythromycin is 80%, 100% and 100% respectively, to trimethoprim sulphate is 48%, 50% and 50% respectively, to doxycycline is 40%, 50% and 50% respectively, to levofloxacin is 24%, 50% and 50% respectively, to nitrofurantoin is 44%, 50% and 50% respectively. 9 stool samples were collected from human, dog and cat examined for presence of specific16s rRNA gene for H. pylori by nested PCR. The positive samples were subjected to Helicobacter genus- specific 16s r RNA gene sequencing.