The present study was established to estimate Cephradine residues in the serum and tissue of broiler chicks following oral administration of Cephradine (Atocef Forte®) and also to detect the effect of potassium citrate on Cephradine residues in broiler chicks. A total number of 40 Hubbard chicks of 21 days old were used in this study after dividing into 2 equal groups (each of 20 chicks). Cephradine (Atocef forte®) was given an oral dose of 15mg\kg body weight twice daily for five consecutive days to the group (1) and was given twice daily for 3 days but at last 2 days, Cephradine was given for 12hr, potassium citrate for another 12h in the group (2). Cephradine was estimated in serum, liver, kidney, and breast muscle of broiler chicks at 24, 48, 72, 96hr post the last dose by using HPTLC assay. Residues study revealed a good absorption and distribution of Cephradine in the liver, kidney and, breast muscle. Cephradine concentrations could be detected in serum till 2d day, in breast muscle till 3d day but in liver and kidney till 4^th day. On the other hand, the group that takes both Cephradine and potassium citrate caused a significant decrease in drug concentration in serum, liver, kidney, and breast muscle.It could be concluded that Cephradine can be detected in the kidney, liver at the highest concentration with the lowest concentration in serum and breast muscle. Potassium citrate is effective in reducing Cephradine residues in broiler chicks.