Canine parvovirus(CPV2) is considered one of the serious and problematic diseases in young puppies, it remains a common and vital reason of morbidity and mortality in puppies, with very low survival rates in untreated dogs. It causes hemorrhagic enteritis and myocarditis in affected dogs. CPV2 has three antigenic variants CPV-2a, CPV-2b and CPV-2c—have been described, which are determined by variations at residue 426 of the VP2 capsid protein. The aim of the present study was to detect CPV-2 in feces of clinically diseased diarrheic puppies by rapid Immunochromatographic test (ICT) followed by polymerase chain reaction (PCR). One hundred fecal samples were collected from clinically suspected dogs with CPV-2 in three different provinces and test by ICT then make extraction of DNA and examined by PCR. The clinical diagnosis was confirmed in 45 suspected clinical cases (45 %) by rapid test (ICT) and 86 % by PCR using common and specific primers sets for detection of CPV2.