A total number of 350 random samples of raw milk, pasteurized milk, dairy products, tap water and water of udder wash were collected from markets in port- Said Province and examined for prevalence of Aeromonas species using starch ampicillin medium, The incidence of Aeromonas species isolated from raw milk, pasteurized milk, kariesh cheese, dmietta cheese, refrigerated white cheese, tap water and water of udder wash were 58%, 26%, 70%, 48%, 40%, 16% and 68%, respectively. The total number of mesophilic Aeromonas isolates from 350 examined samples was 202 isolates of which 104 (51.5%) isolates were A. hydrophila, 60 (29.7%) isolates were A. caviae, 25 (12.4%) isolates were A. sobria and 11 (5.4%) isolates were A. schubertii. The highest number of isolates (42) were recovered from water of udder wash samples, while the lowest number of isolates were recovered from pasteurized milk and tap water samples. Result of antibiotic sensitivity test revealed high degree of sensitivity towards chloramphenicol, amikacin, ceftriaxone and ciprofloxacin, resistant to ampicillin and colistin sulphate. Result of agarose gel electrophoresis revealed that A. hydrophila , A. caviae and A. schubertii have only one plasmid DNA with molecular weight 2.8 kbp While A. sobria was negative for plasmid.