In this study bacteriological examination of 1200 samples were collected from 200 birds (60 recently dead, 80 diseased and 60 apparent healthy broilers). The results revealed that E. coli isolates were recovered from 842 samples with overall prevalence 70.16.  Incidence of positive E.coli from fresh heart blood samples was 75%, liver 83%, kidney 64%, spleen 57%, small intestine74.5% and   from bone marrow 67.5 % . Serological identification of randomly selected E. coli (20) isolates clarified that, one E. coli isolate was serotype O111, one was O44, one was O55, two were serotyped O142, two were serotyped O128, two were serotyped O158, three were serotyped O157, four were serotyped  O29 and four were serotyped O115. PCR assay was carried out on six serovars (O142, O29, O115, O158, O128 and O157) to detect the presence of phoA, iss and iutA gene. All serovars have the three genes except (O29) does not possess iss gene.