Due to the clinical benefits of laparoscopic cholecystectomy over the open procedures 6 adult Saudi Arabian goats have been underwent the work of this study to evaluate the liver function tests before and after Laparoscopic cholecystectomy. Protocol of anaesthesia including intravenous protocol 4mg/kg B.W for introducing endotracheal intubation then anesthesia maintained using Isoflurane. Pneumoperitoneum with CO2 (13-15 mmHg) was used in all animals. Blood samples were taken at baseline (preoperatively), two hours, 12 hours, 36 hours 3 days and 7 days postoperatively to measure liver enzymes and some biochemical parameters. All measured enzymes and other biochemical parameter significantly changed after laparoscopic cholecystectomy and returned around their normal values 7 days postoperatively. It could be concluded that laparoscopic cholecystectomy in goat is a safe and successful procedures even with the usage of relatively high intra abdominal pressure.