A total number of 60 Clarias gariepinus fish obtained from Ismailia governorate and its tributaries were collected from three locations. The fish were divided into three main groups, (group A) from El-Teraa, (group B) from El-Berkaa, (group C) from El-Rashah. These locations derived from Mohamed Ali channel which derived from River Nile. The fish and water of control group were obtained from central laboratory for Aquaculture Research, El-Abbassa, Abo-Hamad, Sharqia, Egypt. Water analysis of the examined polluted locations revealed high level of ammonia. Serum biochemical examinations revealed hypoproteinemia, hypoalbuminemia and hypoglobulinemia with increase in serum ALT, AST, total bilirubin, direct bilirubin, indirect bilirubin, glucose, urea, creatinine and serum ammonia level in the three groups compared with control one.