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65029

Diagnostic Investigation of Salmonella in Captive Budgerigars Using Standard Microbiological Techniques and invA Gene Specific PCR

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

Wildlife and Zoo Medicine

Abstract

A total of 384 faecal samples were collected from apparently healthy budgerigars and processed to investigate the status of Salmonella in captive budgerigars by Standard Microbiological Techniques (SMT) and PCR with invA gene. (10.42%) were Salmonella positive by SMT while (18.49%) were positive by PCR. The incidence by SMT was (11.31%, 12.50% and 6.25%) while, by PCR (25%, 16.67% and 9.38%) in zoos, pet shops and household groups, respectively. The sensitivity and specificity of the PCR were (100% and 91%) respectively. Individual samples that examined by SMT and PCR were compared with Pooling method which considered as initial screening method that eliminates all Salmonella-negative samples. Enriched faecal broth and PCR with invA gene can be used as rapid method for direct detection of Salmonella in the faecal samples of carrier captive birds. Salmonella isolation rate was (87.5%, 7.5%, 5% and 0%) in Spring, Summer, Winter and Autumn respectively.

DOI

10.21608/scvmj.2015.65029

Keywords

Salmonella, nvA Gene, PCR, Captive Budgerigars

Authors

First Name

Enas

Last Name

Saad

MiddleName

-

Affiliation

Wildlife Management and Zoo Medicine Dept. ,Faculty of Veterinary Medicine. Suez Canal University.

Email

-

City

-

Orcid

-

First Name

Atef

Last Name

Kamel

MiddleName

-

Affiliation

Wildlife Management and Zoo Medicine Dept. ,Faculty of Veterinary Medicine. Suez Canal University.

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Khafagy

MiddleName

-

Affiliation

Microbiology Dept. ,Faculty of Veterinary Medicine. Suez Canal University.

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Gamal Eldein

MiddleName

-

Affiliation

Wildlife Management and Zoo Medicine Dept. ,Faculty of Veterinary Medicine. Suez Canal University.

Email

-

City

-

Orcid

-

Volume

20

Article Issue

1

Related Issue

9826

Issue Date

2015-06-01

Receive Date

2015-03-17

Publish Date

2015-06-30

Page Start

147

Page End

156

Print ISSN

1110-6298

Online ISSN

2682-3284

Link

https://scvmj.journals.ekb.eg/article_65029.html

Detail API

https://scvmj.journals.ekb.eg/service?article_code=65029

Order

11

Type

Original Article

Type Code

992

Publication Type

Journal

Publication Title

Suez Canal Veterinary Medical Journal. SCVMJ

Publication Link

https://scvmj.journals.ekb.eg/

MainTitle

Diagnostic Investigation of Salmonella in Captive Budgerigars Using Standard Microbiological Techniques and invA Gene Specific PCR

Details

Type

Article

Created At

22 Jan 2023