The present work was carried out for serological and molecular screening of virulence genes associated with Salmonella in captive budgerigars. A total of 805 apparently healthy birds were collected from different sources, and subjected to clinical and bacteriological examination. Salmonella species were isolated at rate of (4.97%) with recognition of 4 different serovars in which Salmonella Paratyphi A was the most common isolated serotype. All isolates were highly sensitive to Ciprofloxacin, Enrofloxacin and Norfloxacin. Multiplex-PCR using (invA, spvC and stn) was devised to confirm the isolates and predict their virulence. (invA;284bp) was detected in all Salmonella isolates with (100%), while (stn;617bp)and (spvC; 392bp) were detected in some Salmonella isolates.