In Egypt, many challenges facing eradication strategy of FMD concerning the short term immunity induced by inactivated polyvalent FMD vaccine. The aim of the study was directed to ascertain whether post vaccination antibodies against FMDV in buffaloes could be enhanced by administration with levamisole. To achieve the goal, 24 buffaloes were divided into 4 groups, G1 is non vaccinated non adjuvanted group, G2 is vaccine control group, G3 is simultaneously FMD vaccinated and levamisole stimulated group and G4 is FMD vaccinated 7 days after levamisole stimulation. Blood samples were examined for 16 succesive weeks post vaccination for FMD antibodies to serotypes O, A and SAT-2 by ELISA test. The results revealed that animalssimultinously vaccinated and treated with levamisole was higher than the group adjuvinated with levamisole 7 days before FMD vaccination. Levamisole has stimulant effect on the vaccinalimmunity of buffaloes to FMD serotypes O, A and SAT2. Antibody titers of FMD in animal groups were parallel in each group with a high titer in serotype A than O and SAT2. Further investigations are needed to explore the effect of levamisole on cell mediated immune response and challenge test in buffaloes.