This study was conducted to compare between the Mycoplasma gallisepticum and Mycoplasma synoviae in migratory and captive quails. Fifty four (13.5%) and 87 (21.75%) Mycoplasma strain isolates were isolated from 400 samples from migratory and captive quails respectively. Ten (2.5%) Mycoplasma gallisepticum strains were isolated from captive quails by culture method and identified by PCR while, 9 (2.25%) Mycoplasma gallisepticum strains were isolated from migratory quails by culture method but 11 (2.75%) strains were identified by PCR. Mycoplasma synoviae couldn't be isolated by culture method or PCR. The most effective antibiotic was Tylosin which could inhibit the growth of 88.88% of Mycoplasma gallisepticum isolated in case of migratory quails and 80% in case of captive quails using MIC Technique.