Three hundred samples of infected and freshly dead chickens from different farms gathered in Hehia city El-sharkia  for  bacteriological examination  to  detect the presence of Salmonella, 23 Salmonella isolates were detected from 300 samples.Serological results  detected Salmonella enteritidis  with percentage 26.1 % , followed by Salmonella typhimurium  with percentage 17.4 %  and Salmonella kentucky 8.7% . All Salmonella isolates were tested for their susceptibility to the following antimicrobial agents: gentamicin(CN), ,ciprofloxacin (CIP), amoxicillin- clavulanic acid (AMC), , doxycyclin (DO), chloramphenicol (C), erythromycin (E), sulfamethoxazole -trimethoprim (SXT)  High rate of susceptibility was the most common finding obtained against ciprofloxacin (75%)   as shown in table (24). Also, absolute resistance was obtained among Salmonella isolates against erythromycin (100%) and amoxicillin clavulanic acid (100%),  and sulfamethoxazole -trimethoprim (25%).In addition, 33.3% of  isolates were resistant to chloramphenicol, and colistin sulfate. All isolates were resistant to at least three antibiotics and multidrug resistance was seen .PCR detected 6 types of antibiotic resistant genes (aadB gene, qnrS gene ,sul1 gene , floR gene ,dfrA gene and blaTEM  gene) in percentage  91.7% ,83.3% ,66.7%, 75%,33.3% and 83.3% respectively .