Ten bursal samples were pooled from the IBD infected birds in Damietta Governorate showing haemorrhagic and enlarged bursae. Bursal homogenates were used for ten-day-old embryonated specific pathogen free embryonated tests included VN and FAT tests revealed the incidence of IBDV antigen.
Propagation of the obtained isolate in ECE and Vero cells for 10 passages revealed gradual increase in the virus titer up to 8 log10EID₅₀/ml and 7.5TCID₅₀/ml respectively.
Original, egg adapted, cell culture adapted and bursavac samples were used for viral RNA extraction by using reagent---- (Trizol). RT-PCR was performed for samples using V1 and V2 pair of primers of hypervariable region of VP2 gene to confirm the presence of IBDV in cell culture. The results of Viral RNA extracted from all samples is generation of a targeted amplicon of 472 bp confirming that there were no differences between any of them.