During the last two decades, the frequency of fungal infections increased with serious infections. Among these fungi, Candida species was the predominant yeast infection of human and animals. The predominant Candida spp. isolated from human and animals were C.albicans but, the incidence of NAC Candida has been increased. In this study, a total of 205 samples were collected from different human and animal sources. The isolates were identified by phenotypic, commercial biochemical API 20 C AUX kit and RFLP PCR and it was found that the RFLP PCR was the rapid, accurate and cost effective method of NAC identification. The most effective antifungal drug was amphotricin B and some isolates show resistance to common antifungal drugs as fluconazole.