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62742

Prevalence and Molecular Characterization of Pseudomonas Species Isolated From Fish Markets in Port-Said

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

Bacteriology, Immunology and Mycology

Abstract

Total number of 200 apparently healthy fish, Mugil cephalus and Oreochromis niloticus100 of each fish were collected randomly from different markets in Port-Said city to isolate and identify Pseudomonas spp < /em>. from it and the antibiotic sensitivity pattern of isolated strains. Pseudomonas was found in (66%) of Mugil cephalus and (80%) of Oreochromis niloticus. Four hundred and thirty six isolates identified as Pseudomonas spp < /em>. and classified into P. fluorescens (29.13%), P. aeruginosa (27.06%), P. putida (11.01%), P.cepacia (10.55%), P. stutzeri (8.49%), P. anguilliseptica (5.73%), P. alcaligenes (4.13%) and P. acidovorans (3.90%). The identification of Pseudomonas isolates were confirmed by Polymerase Chain Reaction which revealed that tested isolates were Pseudomonas and produced specific band at 618 bp, P. fluorescens produced specific band at 850 bp and P. aeruginosa produced electrophoresis at 956 bp. Antibiogram results showed that all Pseudomonas isolates were highly sensitive to Ciprofloxacin, Gentamicin and Chloramphenicol while were highly resistant to Ampicillin/sulbactam, Penicillin and Amoxicillin.

DOI

10.21608/scvmj.2016.62742

Keywords

Molecular characterization, Pseudomonas Species, Fish Markets, Port-Said

Authors

First Name

Hamza

Last Name

Eid

MiddleName

-

Affiliation

Bacteriology, Immunology and Mycology Department, Fac. Vet. Med. Suez Canal University,

Email

-

City

-

Orcid

-

First Name

Azza

Last Name

El Tabiy

MiddleName

-

Affiliation

Bacteriology, Immunology and Mycology Department, Fac. Vet. Med. Suez Canal University

Email

-

City

-

Orcid

-

First Name

Sara

Last Name

Fathy

MiddleName

-

Affiliation

Animal Health research institute, Port-Said branch.

Email

-

City

-

Orcid

-

Volume

21

Article Issue

1

Related Issue

9510

Issue Date

2016-06-01

Receive Date

2016-01-21

Publish Date

2016-06-30

Page Start

1

Page End

12

Print ISSN

1110-6298

Online ISSN

2682-3284

Link

https://scvmj.journals.ekb.eg/article_62742.html

Detail API

https://scvmj.journals.ekb.eg/service?article_code=62742

Order

1

Type

Original Article

Type Code

992

Publication Type

Journal

Publication Title

Suez Canal Veterinary Medical Journal. SCVMJ

Publication Link

https://scvmj.journals.ekb.eg/

MainTitle

Prevalence and Molecular Characterization of Pseudomonas Species Isolated From Fish Markets in Port-Said

Details

Type

Article

Created At

22 Jan 2023