The present study was undertaken during a period from2013 to 2015 in different localities at Damietta Governorateto investigate the isolation prevalence of Enterococcusspp., antibacterial resistance patterensand their pathogenicity in Pekin ducks . Clinical cases from 46 duck flocks of  different breedssuspected with bacterial infection (26 farms and 20backyard),were examined for the prevalence ,isolation and identification.  Enterococcus faecalis. Suspected cases were confirmed after culturing on Bile esculinazid agar, biochemical testing either by conventional and bioMerieuxVitek. Prevalence were (7/26) and   (4/20) in duckling farms and backyard respectively.Sensitivity testing of isolated enterococci from duck flocks to antimicrobial agents were tested using the disk diffusion method.Enterococcus faecalis showed multiple drug resistance patterns for different antibiotics ,oxytetracyclin, chloramphenicol, Erythromycin, Gentamicin, Ciprofloxacin, Neomycin, Kanamycin, Vancomycin, Nalidixic acid, and streptomycin while,  susceptible to Ampicilin.Pathogencity test for isolated   Enterococcus fecalis was performed .Inoculated ducks showed mainly,general clinical signs respiratory manifestation, lameness , growth retardation and pathologically airsaculitis, perihepatitis, pericarditis ,endocarditis, splenomegaly and necrosis of bone extremities (FHN) differ in severity according to the route of inoculation intravenous, intrathoracic, intranasal and orally respectively.