Hyperimmune serum against peste des petites ruminants virus was successfully prepared in horses where it was found to have specific peste des petites ruminants virus (PPRV) neutralizing antibodies titer of 1024/ml as determined by serum neutralization test (SNT). Quality control testing of such serum revealed that it was free from bacterial, fungal and mycoplasma contaminants as tested on specific media and safe when inoculated in sheep. Passive induced immunity in sheep was persisted with a protective level up to 5 weeks post inoculation as determined by SNT and ELISA. On the other side, inoculation of PPR vaccine with antisera in sheep showed similar findings with slight rise in antibody titer. Depending on the obtained results, it could be concluded that horse anti-PPR serum is of a significant importance and can help to protect and control PPR infections especially in case of outbreaks that need rapid management.