Infectious laryngotracheitis (ILT) is an economically important disease of chickens caused by type 1gallidherpesvirus (infectious laryngotracheitis virus, ILTV). Samples from lungs, trachea, liver and spleen were taken, representing 20 farms at Ismailia Province, for molecular studies and histopathological examination on Infectious Laryngotracheitis virus. The samples were obtained from broiler Saso chicken (50 days old) and breeders (65-100 day old) with variable morbidities and mortalities. Four pooled samples from the 20 farms were examined for characterization and identification of ILT virus by PCR. Electrophoretic patterns showed a specific band at 688bp positive in two samples while it was negative in the other two samples. Samples were genetically characterized as Gallid herpesvirus 1 with similarity to ILTV/Brazil/2007/USP-65 ICP4 by 98%Gen bank acc. no: gb|FJ477379.1|.The charactrestic histopathological lesions were found in the lung and trachea. The lung showed severe congestion emphysema and collapse. The trachea revealed degeneration and sloughing of tracheal epithelium with  hyperemia, edema and leuckocytic infiltrations in the lamina propria and  submucosa. The detected virus in this study was genetically characterized as Gallid herpes virus 1 with similarity to ILTV/Brazil/2007/USP ICP4 by 98%. For the prevention on latent infection need to improve recombinant vaccines. The PCR study proved the occurance of ILTV in the respiratory disease complex (RDC) at Ismailia poultry flocks. Recombinant vaccine improvement is recommended as prophylactive measures for the latent infections among chickens.