Staphylococcus aureus is a frequent cause of infections in both the community and hospital. Methicillin-resistant Staphylococcus aureus  is a common nosocomial pathogen that causes the infections in different department of the hospital. Present study was conducted in Ismailia General Hospital to screen the nasal carriage of Methicilin resistant Staphylococcus aureus  among  health care worker who have contact with patients  to help in control the spread of this pathogen from the health care workers to the patients.  A total of 400 samples: 230 nasal swabs from healthy medical staff and worker and 150 patients' clinical specimens and 20 environmental swabs were subjected to bacteriological investigation following standard protocol. Sixty two isolates belong to S. aureus from 400 isolates. Out of twenty eight isolates were Methicillin-resistant Staphylococcus aureus.  Antimicrobial susceptibility test was performed by disk diffusion method as per the CLSI guidelines. MecA gene was identified for isolates at 310bp.  Genotyping by PCR-RFLP of Coa and Spa gene was identified for MRSA isolates has a special role in assessing the relatedness of MRSA isolates and its control.
Genotyping of MRSA by Coa and Spa Gene Polymorphism among Nasal Carriage of  Health Care Workers and Patients Clinical Isolates at Ismailia General hospital
Serry F. M., Kadry A. A. and. Mansour M. K.
Microbiology and Immunology Department, Faculty of Pharmacy, Zagazig University
 
Abstract
Staphylococcus aureus is a frequent cause of infections in both the community and hospital. Methicillin-resistant Staphylococcus aureus  is a common nosocomial pathogen that causes the infections in different department of the hospital. Present study was conducted in Ismailia General Hospital to screen the nasal carriage of Methicilin resistant Staphylococcus aureus  among  health care worker who have contact with patients  to help in control the spread of this pathogen from the health care workers to the patients.  A total of 400 samples: 230 nasal swabs from healthy medical staff and worker and 150 patients' clinical specimens and 20 environmental swabs were subjected to bacteriological investigation following standard protocol. Sixty two isolates belong to S. aureus from 400 isolates. Out of twenty eight isolates were Methicillin-resistant Staphylococcus aureus.  Antimicrobial susceptibility test was performed by disk diffusion method as per the CLSI guidelines. MecA gene was identified for isolates at 310bp.  Genotyping by PCR-RFLP of Coa and Spa gene was identified for MRSA isolates has a special role in assessing the relatedness of MRSA isolates and its control.