A total of 300 chicken samples (100 samples for breast muscles, 100 samples for thigh muscles and 100 samples for joints) were collected randomly from different markets in Port-said city during the period of work from July 2015 till August 2016. Overall percentage of coagulase positive S. aureus which isolated from chicken samples were 37.2% (49/132) while coagulase positive S. aureus of breast muscles, thigh muscles and joints were 15.1%, 14.5% and 7.6% respectively. PCR revealed that 15 tested isolates were S. aureus using 16SrRNA at 791 bp. The study conducted to detect virulence genes, coa 15 isolates were positive at 570 bp. (100%),  clfA15 isolates were positive at 638 bp. (100%),  See gene is the highest SE, 5 isolates were positive at 209 bp. (33.33%), followed by Seb gene 1isolate were positive at164 bp. (6.67%). None of 15 isolates were positive for Sea, Sec and Sed.