This study was designed to characterize antifungal susceptibility pattern of different species of dermatophytes isolated from human and animal dermatophytosis against six commercially available antifungal agents recommended for treatment. Broth microdilution method was used to determine minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of different drugs against the tested dermatophyte species. MIC was done against fluconazole, itraconazole, terbinafine, tioconazole, miconazole and griseofulvin.  Of the antifungal agents tested, the best results in terms of sensitivity were found with terbinafine for all isolates and tioconazole, miconazole   for M. canis and T. verrucosum while the antifungal activity of fluconazole was found to be weak. Routine detection of MIC for antifungal agents serves as a rapid and reliable method for treatment of human and animal dermatophytosis.