Dermatophytes are keratinophilic fungi that infect keratinized tissues causing diseases known as dermatophytoses. Dermatophytes are classified in three genera, Epidermophyton, Microsporum, and Trichophyton. This investigation was performed to study the prevalence of dermatomycosis among 200 samples were collected from human and some animal species (100 samples from each of human and animal), human samples were collected from skin diseases center at Ismailia clinic while animal samples were collected from El-Salhia farm, Internal medicine clinic Faculty of Veterinary Medicine, Suez Canal University in Ismailia, Al- Zahraa farm and private pet animal clinics in cairo. The skin scraping and hair samples were obtained aseptically by plucking hair with forceps around the affected area and scraping the epidermal scales with a sterile scalpel blade. All collected samples were examined by direct microscopy and culture technique. Laboratory identification of the fungal isolates was based on their colonial and microscopic characteristics. Dermatophytes were isolated in a percentage 40% in human samples while the percentage was 61% in animal samples. The most common isolated dermatophyte species in human were M. canis, T. violaceum and T. mentagrophytes, in pets was M. canis, in cattle was T. verrucosum and in horse were T. mentagrophytes and T. verrucosum