Salmonellosis has been considered as one of the most important infectious diseases in humans and animals. The present study reports traditional methods for characterization of Salmonella enterica from different sources. Two hundred seventy samples were collected from different poultry farms at Sharkia Governorate during the period from November 2015 to May 2016, and subjected to traditional bacteriological examination for isolation of Salmonella. Incidence of Salmonella from fecal swabs and internal organs revealed the isolation of Salmonellawith an overall incidence 14.07% (38/270). Absolute resistance was observed for amoxicillin/clavulanic acid, ampicillin, doxycycline and ceftriaxone On the other hand, sensitivity to ciprofloxacin (81.6%), gentamicin (78.9%) ,sulphamethoxazol/trimethoprim (73.7%),amoxicillin (65.8%)  and  chloramphenicol(63.2%), Serotyping of Salmonella revealed two Salmonella serogroups. The first is Salmonella Typhimurium with antigenic formula 1,4,5,12:i:1,2 as (6/8) by (75 %) and Salmonella Lagos with antigenic formula 1,4,5,12:i:1,5 as (2/8) by (25%). These findings illustrated that all serotypes were resistant to ceftriaxone, doxycycline, ampicillin and Amoxicillin/clavulanic acid. while, ciprofloxacin gave high degree of sensitivity, the  outbreak of infection in poultry is not only by common type such as S. Typhimurium but also by other serotypes such as S. lagos.