A total number of 350 cultured and wild freshwater fishes of different body weights. They were randomly collected from kafer El-sheikh cultured fish farms and also from various water sourses for detection of blood protozoal diseases. The collected fishes were represented as 100 wild Clarias gariepinus, 100 wild Oreochromis niloticus, 75 cultured O. niloticus and 75 cultured Cyprinus carpio. The cultured and wild fishes were collected seasonally. The main clinical picture of the infested fishes with blood protozoal parasites were abrasions on the external body surface, off food, emaciation, dullness, respiratory manifestation. Pale  gills and external body surface with distended abdomen . The total prevalence of blood parasites in all examined fishes was 29.7 %. The highest rate of infestation of Trypanosoma mukasai and Babesioma clarias wasin wild C. gariepinius. Babesioma tilapae in cultured & wild tilapia and Babesioma sp.in cultured C. carpio showedthe highest infestation in summer, summer and spring seasons respectively. Protozoal blood parasites showed dramatic changes in haemogram, leukogram picture, total proteins, albumin, globulin blood level, glucose and creatinine on all examined fishes. The infected fishes with Trypanosoma and Babesioma sp. showed different histopathological changes in liver, kidneys, spleen, gills, skin and musculature.