Various sectors of the aquaculture industry can benefit if cultured organisms were conferred with enhanced growth performance, feed efficiency and disease resistance. As such, the cost of medication and production costs could be reduced and consumer perceptions would be improved.  The objectives of the present study were to investigate the effects of sorghum and inulin on ectoparasitic infection and protein gel electrophoresis in fingerlings of Nile tilapia (Oreochromis niloticus) with initial body weight of 9.2±0.037g. Six diets were formulated by using sorghum as source of carbohydrate incorporated as two levels (15 and 30%) of the diet supplemented with inulin at three doses (2.5, 5, 7.5g). Tilapias fed inulin containing diets (2.5 g) with sorghum (15 and 30%) exhibited significant improvements of immune system of fish and increased resistance of infection. The morbidity rate decreased after cohabitation with parasitic fish in low dose of inulin also number of protein bands increased of protein gel electrophoresis. Inulin in high doses (5 and 7.5g) revealed reverse effect of Cichlidogyrus tilapae infection and number of protein bands