Campylobacteriosis is one of the main common food borne illnesses all over the world causing gastroenteritis in human. So, this study aimed to make genotyping of Campylobacter spp. isolated from fecal samples of both broiler and human with detection of tetracycline resistance strains. A total of 100 Campylobacter spp. previously identified and confirmed by presence of 23SrRNA gene from broiler and human fecal samples (50 of each) were subjected to duplex conventional PCR for detection of mapA gene which specific for C. jejuni and ceuE gene for C. coli. Tweenty randomly selected Campylobacter strains (10 from C. coli and 10 from C. jejuni) were examined for presence of TetO gene which responsible for tetracycline resistance. Results revealed that, the total percentages of C. coli and C. jejuni were 76% and 24% respectively. C. jejuni was 14 % while C. coli was 86% in fecal samples collected from broiler, Whilefecal samples collected from human, the percentage of C. jejuni was 34% while C. coli was 66%. All 20 recovered strains showed completely resistance to tetracycline with percentage 100%, which is considered the first record in Ismailia, Egypt. This study gives insight about the importance of continuous survey of Campylobacter strains with great attention to developing of newly antimicrobial resistance strains.