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200558

Antioxidant and Cytotoxicity Potential of Piperine and Sorafenib combination in human MDA-MB-231 Breast Cancer cells

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Last updated: 24 Dec 2024

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Abstract

Background: Breast cancer is the most common cancer diagnosed in women. Worldwide, it is the second most common cause of cancer-related mortality among women. Plants have been the primary sources of natural product drug discovery, over 60% of the current anticancer drugs were derived from natural sources. Piperine (PIP) is a major bioactive constituent of the black pepper (Piper nigrum). Aim: We have assessed  the cytotoxicity of PIP and the anti-hepatocellular carcinoma drug Sorafenib (SOR) against human triple negative breast cancer MDA-MB-321 cell line. Furthermore, we have investigated the potential effect of single and their combined treatment on antioxidant enzyme activity and lipid peroxidation status. Methods: We measured cytotoxicity of PIP and SOR in MDA-MB-321 cells by MTT assay after 48 hours treatment. Activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and the lipid peroxidation marker Malondialdehyde (MDA) were measured colorimetrically. Results: Both PIP and SOR caused a significant (P<0.001) dose dependent cell death in MDA-MB-321 cells. However, SOR showed higher cytotoxicity (IC90= 144.8 ± 5.1µg/ml) compared to PIP (IC90= 252.4 ± 6.7µg/ml). Consistent with these data, SOR individual treatment caused the highest significant SOD and CAT activities while their combined treatment caused the lowest MDA levels (4.2 ± 0.2 nmol/ml) compared with the control untreated (7.7 ± 0.2 nmol/ml) or DMSO treated cells (7.76 ± 0.25 nmol/ml). Conclusion: This study points out a potential mode of action for PIP and SOR as anticancer agents in triple negative breast cancer cells through the regulation of their antioxidant and lipid peroxidation status.

DOI

10.21608/blj.2021.200558

Keywords

Piperine, Sorafenib, Lipid peroxidation, antioxidants, breast cancer

Authors

First Name

Hany

Last Name

Mohany

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Affiliation

Department of Chemistry, Faculty of Science, Kafr el sheikh University.

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First Name

Mohammad A.

Last Name

Mahmoud

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Affiliation

Department of Chemistry, Faculty of Science, Kafr el sheikh University.

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First Name

Ashraf A.

Last Name

El-Shehawy

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Affiliation

Department of Chemistry, Faculty of Science, Kafr el sheikh University.

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First Name

Mohamed

Last Name

Gaber

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Affiliation

Chemistry Department, Faculty of Science, Tanta University

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Orcid

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First Name

Alaa

Last Name

Elmetwalli

MiddleName

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Affiliation

Department of Clinical Trial Research Unit and Drug Discovery, Egyptian Liver Research Institute and Hospital (ELRIAH), Mansoura, Egypt.

Email

dr.prof2011@gmail.com

City

-

Orcid

0000-0001-5372-4297

First Name

Afrah F.

Last Name

Salama

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-

Affiliation

Biochemistry Section, Chemistry Department, Faculty of Science, Tanta University.

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Orcid

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First Name

Tarek

Last Name

El Sewedy

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Affiliation

Department of Applied Medical Chemistry, Medical Research Institute, Alexandria University

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Volume

17

Article Issue

1

Related Issue

26011

Issue Date

2021-12-01

Receive Date

2021-09-14

Publish Date

2021-12-01

Page Start

108

Page End

116

Print ISSN

1687-4773

Online ISSN

2974-4725

Link

https://blj.journals.ekb.eg/article_200558.html

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https://blj.journals.ekb.eg/service?article_code=200558

Order

10

Type

Original Article

Type Code

988

Publication Type

Journal

Publication Title

Biochemistry Letters

Publication Link

https://blj.journals.ekb.eg/

MainTitle

Antioxidant and Cytotoxicity Potential of Piperine and Sorafenib combination in human MDA-MB-231 Breast Cancer cells

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Article

Created At

22 Jan 2023