Background: Natural products have been known as one of the most important therapeutic agents for diabetes and hyperlipidemia. Bee venom is a multipurpose agent that contains different bioactive compounds including melittin and phospholipase A2. Aim: The aim of this study was to investigate the effects of bee venom on biochemical and histopathological abnormalities in pancreas, liver and kidney of diabetic hyperlipidemic rats compared to synthetic drugs (Metformin and Atorvastatin).Material and methods: Bee venom's median lethal dose (LD50) was determined, and then 50 adult male albino rats were divided into five groups: group 1 was fed standard diet and served as a negative control group, while the other four groups were received streptozotocin and nicotinamide injections to induce type 2 diabetes and after diabetes confirmation, rats were fed a high-fat diet for 28 days and then they were divided as follows: group 2 : served as a positive control group, and the remaining three groups represented the treated groups, group 3: bee venom treated group (0.5mg/kg), group 4: bee venom treated group (1/10 LD50) (1.23mg/kg), and group 5: Metformin (60 mg/kg) plus Atorvastatin (10 mg/kg) treated group for 28 days, respectively. At the end of the experiment: blood samples, liver, kidney, and pancreas tissues were collected. Results: Treatment of diabetic hyperlipidemic rats using two doses of bee venom (0.5 and 1.23 mg/kg) and Metformin plus Atorvastatin revealed significant decrease (p < 0.0001) in levels of glucose, HOMA-IR, total protein, globulin, blood urea nitrogen, creatinine, MDA, Fetuin-,A, ALT and AST activities compared to the positive control group. Furthermore, Levels of insulin, HOMA-ß, Albumin, A/G ratio, and TAC were significantly increased compared to the positive control group (p < 0.0001). Our results were confirmed by histopathological examination of the pancreas, liver, and kidney tissues. Conclusion: Bee venom can be considered as a new potential therapeutic strategy for diabetes associated with hyperlipidemia.