Background: Rheumatoid arthritis (RA) was characterized by various systemic inflammation of joints causing damage of bone and cartilage. Aim: The current study aimed to invistigae serum level of vitamin D and calcium in RA patients compared to healthy control. Materials &Methods: This study included 90 individuals divided into two groups: Healthy volunteers group (15) and RA group (75) from Rheumatology and Rehabilitation Department, Faculty of Medicine, Zagazig university hospitals. Vitamin D and calcium levels in sera were measured as patients consumed calcium tablets. Erythrocyte sedimentation rate (ESR), C - reactive protein (CRP), Rheumatoid Factor (RF), Hemoglobin percentage (HB %) and platelet count were collected from data sheet of patient. Results: There were no significant differences between Vitamin D, calcium (slightly increased in patients) and hemoglobin in both groups. While there were a significant difference in ESR, CRP, RF, and platelet count between RA and healthy control as (p=<0.0001), (p=0.010), (p=0.015) and (p=0.03) respectively.  Conclusion: Vitamin D and calcium showed non-significant difference between patients and control.