Background & Objectives: Hepatic fibrosis is the extreme extracellular matrix buildup of collagen due to the chronic liver disorders. Progressive liver fibrosis results in cirrhosis and hepatic failure. Nilotinib is considered a 2^nd generation tyrosine kinase inhibitor, which exhibited antifibrotic efficacy. Stem cells release cytokines and growth factors which exhibited paracrine-mediated antifibrotic and anti-inflammatory effects in vitro and in vivo. Hence, stem cells conditioned medium (SC-CM) may have an antifibrotic role. This study is designed to explain the association between the oxidative stress markers and the hydroxyproline content in liver fibrosis. Methods: Sixty male wistar rats were distributed into six major groups; control group, CCl₄ group, Free conditioned media group, (CCl₄ & Nilotinib) group, (CCl₄ & stem cells exosomes) group, and (CCl₄ & Nilotinib & stem cells exosomes) group. They had an intraperitoneal injection with CCl₄ for nine weeks and had treatments of Nilotinib, stem cell exosomes (extracted from Liver mesenchymal stem cells), and a combined treatment of Nilotinib and stem cell exosomes from the 5^th week to the 9^th week of CCl₄ intoxication. Oxidative stress parameters (e.g. MDA, SOD, GSH, CAT, NO, and NOS) and hydroxyproline content were estimated for each group. Results: results showed a significant positive correlation between Hydroxyproline and MDA, NO and NOS. Also, there is a significant negative correlation between Hydroxyproline and GSH, SOD and CAT. Conclusion: There is a significant positive correlation between Hydroxyproline and MDA, NO and NOS. And there is a significant negative correlation between Hydroxyproline and GSH, SOD, CAT.