Background: This study aimed to evaluate the ability of mesenchymal stem to treat liver injury. Liver transplantation and surgical treatment may be one of the good available solutions for liver injuries. However, it is painful and limited due to shortage of donor organs and high medical costs. Mesenchymal stem cells (MSCs) can serve as an autologous treatment to the liver injury. This study aims to evaluate the ability of MSCs to treat liver injury. Rat MSCs were isolated, expanded for 4 passages. Then they were injected into the tail vein of Sprague Dawley rats which were previously inducted to liver fibrosis with carbon tetrachloride (CCL₄). After and before cell injection, the biochemical analysis of liver function tests and the immunohistochemistry were tested for the liver tissue after 6 weeks of disease induction. Conclusion: AD-MSCs have a promising effect against CCL4 induced liver fibrosis as well as enhancing liver function tests.