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EFFECT OF GLUCOCORTICOIDS ON PIT 1 INHIBITION OF SV40 ENHANCER ACTIVITY

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Last updated: 04 Jan 2025

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Abstract

Earlier studies had shown an effect of glucocorticoids receptor on Pit 1 activation of rPrl promoter activity Treacy. Therefore, the effect of glucocorticoids on Pit 1 inhibition of SV40 enhancer function was investigated in a series of co-transfection studies into glucocorticoids receptor deficient CV-1 cells. Under identical conditions co-transfection of CMV – Pit 1 activated transcription from prPrl (-1960-+38) – CAT; however, expression of GR in the presence of dexamethasone (10-6M) suppressed this activation. The effect of glucocorticoids on the inhibition of SV40 enhancer activity by Pit 1 was investigated. In co- transfection studies in CV-1 cells (Figure 4) it was shown that GR expression and the provision of glucocorticoids (dexamethasone 10-6M) blocked the ability of Pit 1 to inhibit SV40 enhancer activity. Under the same conditions the ability of Pit 1 to activate the rPrl promoter (-1960/+38) was inhibited.

DOI

10.21608/blj.2010.64438

Authors

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S.

Last Name

El-Jaafary

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Affiliation

Department of Biochemistry Faculty of Medicine AL-Fateh University Tripoli, S.P.L.A.J

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First Name

A. B.

Last Name

EL Fighi

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Affiliation

Department of Biochemistry Faculty of Medicine AL-Fateh University Tripoli, S.P.L.A.J

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First Name

S. A .B.

Last Name

Hasan

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Affiliation

Department of Biochemistry Faculty of Medicine AL-Fateh University Tripoli, S.P.L.A.J

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Volume

6

Article Issue

1

Related Issue

9732

Issue Date

2010-12-01

Receive Date

2010-06-11

Publish Date

2010-12-01

Page Start

113

Page End

118

Print ISSN

1687-4773

Online ISSN

2974-4725

Article File

BLJ_Volume 6_Issue 1_Pages 113-118.pdf

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https://blj.journals.ekb.eg/article_64438.html

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https://blj.journals.ekb.eg/service?article_code=64438

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Original Article

Type Code

988

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Journal

Publication Title

Biochemistry Letters

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https://blj.journals.ekb.eg/

MainTitle

EFFECT OF GLUCOCORTICOIDS ON PIT 1 INHIBITION OF SV40 ENHANCER ACTIVITY

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Article

Created At

22 Jan 2023