Beta
ManageSearchChatDiscover
64435

INHIBITION BY GLUCOCORTICOIDS OF PIT 1 INDUCED ACTIVATION OF TRANSCRIPTION FROM RPR1 PROMOTER FRAGMENTS IN NON PITUITARY CELLS

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Bovine and rat prolactin gene promoter function by glucocorticoids. It was of interest, therefore to examine the possible inhibition by glucocorticoids of pit l induced activation of transcription from the 3'- rPrl promoter mutant generated for this study. The studies were carried out by co-transfection into glucocorticoids receptor (GR) deficient CV-1 cells both pin1 and (GR) expression vectors and the reporter CAT plasmids with upstream rPrl promoter fragments. The effect of glucocorticoids was examined by the Addition of dexamethasone (10-6 M) to the cultures immediately after transfection. In these studies glucocorticoids receptor (GR) deficient CV-l cells were used and the (GR) was expressed from an expression vector parker et al. (1994). However, in the case of prolactin promoter fragments (eg, - 1960/+ 38, -44/ -423, -190/-423 and – 75 /+ 38) decreased in pitl induced DAT gene expression was seen when the (GR) was expressed but it was evident even in the absence of glucocorticoids.

DOI

10.21608/blj.2010.64435

Authors

First Name

S.

Last Name

Eljaafari

MiddleName

-

Affiliation

Department of biochemistry faculty of medicine Alfateh University

Email

-

City

-

Orcid

-

First Name

A:B.

Last Name

El-Fighi

MiddleName

-

Affiliation

Department of biochemistry faculty of medicine Alfateh University

Email

-

City

-

Orcid

-

First Name

I.M.

Last Name

fahelbum

MiddleName

-

Affiliation

Department of biochemistry faculty of medicine Alfateh University

Email

-

City

-

Orcid

-

Volume

6

Article Issue

1

Related Issue

9732

Issue Date

2010-12-01

Receive Date

2010-06-09

Publish Date

2010-12-01

Page Start

106

Page End

112

Print ISSN

1687-4773

Online ISSN

2974-4725

Article File

BLJ_Volume 6_Issue 1_Pages 106-112.pdf

PDF . 489.5kB

Link

https://blj.journals.ekb.eg/article_64435.html

Detail API

https://blj.journals.ekb.eg/service?article_code=64435

Order

8

Type

Original Article

Type Code

988

Publication Type

Journal

Publication Title

Biochemistry Letters

Publication Link

https://blj.journals.ekb.eg/

MainTitle

INHIBITION BY GLUCOCORTICOIDS OF PIT 1 INDUCED ACTIVATION OF TRANSCRIPTION FROM RPR1 PROMOTER FRAGMENTS IN NON PITUITARY CELLS

Details

Type

Article

Created At

22 Jan 2023